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KMID : 0900920000240020217
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Korean journal of Animal Reproduction
2000 Volume.24 No. 2 p.217 ~ p.222
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Effect of Quiescent Treatment on Nuclear Remodeling and In Vitro Development of Nuclear Transfer Embryos Derived from Bovine Fetal Fibroblast Cells
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Kim Choung-Ik
Park Choon-Keun
Yang Boo-Keun
Cheong Hee-Tae
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Abstract
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This study was conducted to investigate the effect of quiescent treatment of the donor cells on the nuclear remodeling and in vitro development of fetal fibroblast cell-cloned bovine embryos. Serum starved, confluent and nonquiescent cycling fetal fibroblast cells were transferred into the enucleated oocytes. About 20∼25% of nuclear transfer embryos fused with a serum starved or confluent cell extruded a polar body, which was slightly lower than that of nontreated control (36%). About 49∼51% of nuclear transfer embryos fused with a serum starved or confluent cell had a single chromatin clump, which was slightly higher than that of nontreated control (40%). The proportion of embryos with a single chromatin clump was significantly higher (P＜0.01) in nuclear transfer embryos without showing a polar body (60.5%) than with a polar body (4.7%). Development rates to the blastocyst stage were 21.7% and 20.9% when serum starved and confluent cells were transferred, which were slightly higher than that of control (14.1 %). The result of this study suggests that quiescent treatment by serum starvation or growth to confluency of donor cells could increase the number of embryos with a normal chromatin structure, which results in increased in vitro development.
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KEYWORD
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Nuclear transfer, Fetal fibroblast cells, Nuclear remodeling, Quiescent treatment, In vitro development
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